Postdoctoral Researcher · Niyogi Lab

Amala
John

University of California, Berkeley
Dept. of Plant & Microbial Biology

Engineering photosynthesis and soil biology to address climate change through CRISPR genome editing.

Amala John presenting research at a scientific conference

Bridging molecular biology and climate science

I am a postdoctoral researcher in the Niyogi Lab at UC Berkeley's Department of Plant & Microbial Biology, working at the intersection of plant molecular biology and climate change mitigation. My research applies CRISPR genome editing to enhance photosynthetic efficiency and carbon sequestration in plants.

I completed my Ph.D. in the Zachary Nimchuk Lab at UNC Chapel Hill, where I uncovered novel connections between receptor complexes, hormone signaling, and plant meristem development.

My skillset spans molecular biology, confocal and SEM imaging, bioinformatics, and experimental design — paired with a commitment to communicating science across disciplines.

Molecular Biology CRISPR / Genome Editing Confocal Microscopy SEM Imaging Experimental Design RNA-seq & Bioinformatics Plant Transformation Statistical Analysis
Cell Wall
Current Position

Postdoctoral Researcher
Niyogi Lab, UC Berkeley

Ph.D., Biology

University of North Carolina
Chapel Hill

B.S., Molecular Biology (Honors)

UCLA

Research Focus

Photosynthesis · CRISPR
Carbon Sequestration

Current & Past Research

CLV Receptor Signaling & Meristem Development

Nimchuk Lab · UNC Chapel Hill · Ph.D.

Identified a novel connection between the CLV2/CRN receptor complex and flower development, demonstrating how temperature and auxin modulate this process. Used CRISPR in Arabidopsis, confocal microscopy across 14+ reporter lines, and RNA-seq analysis.

DNA Methylation & Folate Metabolism

Jacobsen Lab · UCLA · Undergraduate

Investigated the role of MTHFD1 and folate metabolism in DNA methylation in Arabidopsis. Contributed root growth assay data and BS-Seq library preparation published in Nature Communications.

Plant Breeding & Protoplast Optimization

Syngenta · Industry Internship · 2022

Cloned key vectors for plant breeding optimization via Golden Gate and RE cloning. Optimized a protoplast assay for high-throughput screening; contributed to maize embryo rescue across two cross-functional project teams.

Selected Publications

2022

A network of CLAVATA receptors buffers auxin-dependent meristem maintenance.

John, A., Sarkel S.E., Jones S.D., Soyars L.C., & Nimchuk L.Z.

bioRxiv — Preprint; submitted to Nature Plants

doi:10.1101/2022.05.27.493758
2021

CLAVATA Signaling Ensures Reproductive Development in Plants across Thermal Environments.

Jones S.D., John, A., VanDerMolen R.K., & Nimchuk L.Z.

Current Biology

doi:10.1016/j.cub.2020.10.008
2019

A little goes a long way: CLE peptides mediate phloem initiation.

John, A. & Nimchuk L.Z.

Journal of Integrative Plant Biology

doi:10.1111/jipb.12868
2016

MTHFD1 controls DNA methylation in Arabidopsis.

Groth M., Moissiard G., Wirtz M., ... John, A., ... & Jacobsen S.E.

Nature Communications, 7.

doi:10.1038/ncomms11640

Academic & Professional Journey

Present

Postdoctoral Researcher

Niyogi Lab · UC Berkeley, Dept. Plant & Microbial Biology

Applying CRISPR genome editing to improve photosynthetic efficiency and carbon sequestration in plants, as part of the IGI Carbon Removal initiative funded by Chan Zuckerberg Initiative.

Summer 2022

Reproductive Biology Intern

Syngenta · Durham, North Carolina

Cloned 2 key vectors for plant breeding optimization; optimized protoplast assay for high-throughput screening; contributed to maize embryo rescue and field work.

2016 – 2022

Ph.D. Candidate — Biology

Zachary Nimchuk Lab · UNC Chapel Hill

Investigated CLV receptor signaling in plant meristem development. Developed expertise in CRISPR, confocal & SEM microscopy, and RNA-seq. Published 3 papers; presented at 4 international conferences.

2014 – 2016

Undergraduate Researcher

Steve Jacobsen Lab · UCLA

Uncovered the role of folate metabolism in DNA methylation via MTHFD1. Contributed to root growth assays and BS-Seq library preparation published in Nature Communications.

2012 – 2016

B.S. Molecular, Cell and Developmental Biology (Honors)

University of California, Los Angeles (UCLA)

Selected Talks

  • 2022American Society of Plant Biologists (ASPB) Annual Meeting — Portland, OR
  • 2021Plant Hormone Meeting — Duke, NC
  • 2019Plant Molecular Biology Retreat — Wilmington, NC
  • 2019American Society of Plant Biologists (ASPB) Annual Meeting — San Jose, CA

Get in Touch

Interested in collaborating, discussing research, or connecting? I'd love to hear from you.